If your dog has been relieving itself indoors, you're probably on the hunt for a good enzyme cleaner to eliminate that unwelcome stain and smell. Enzyme cleaners are formulated to break down and remove organic matter, making them ideal for removing pet urine stains and odors. In this article, we'll take a look at some of the best enzyme cleaners for dog urine available on the market today.
We hope you enjoy the following product reviews. All products we evaluate are carefully chosen from among the best currently available. We may, in some cases, receive samples for hands-on evaluation - but all opinions noted in this article are ours alone. As an Amazon Associate, we earn from qualifying purchases made by our readers. Thank you for visiting us today!
How We Pick the Best Enzyme Cleaner for Dog Urine
When it comes to finding the best enzyme cleaner for dog urine, there are a few key factors you'll want to keep in mind. You'll want to make sure that the cleaner is specifically designed for use on pet urine. While many general-purpose enzyme cleaners claim to be effective on pet urine, they're not always up to the task. There are many other things to keep in mind when looking for the best enzyme cleaner for removing pet odors and stains.

Natural Enzymes Feed On Ammonia Crystals & Organic Matter
Rocco & Roxie Stain & Odor Eliminator
What We Like About It
Rocco & Roxie's Enzyme-Powered Pet Odor Eliminator uses enzymes to target and eliminate stains, odors, and residue, leaving your surfaces clean and fresh. It's perfect for use on carpets, floors, furniture, clothing, litter boxes, kennels, carriers, and anywhere else stains happen. It's chlorine-free and color-safe.
The natural enzymes in the spray are activated on contact with odors and stains, immediately going to work to break down the source of the problem. In addition, the 100% satisfaction guarantee means that if you're not completely happy with the results, you can get your money back - no questions asked.
One Minute Pet Tips
Thoroughly saturate the stain with the product. For best results, use an amount of product equal to the size of the urine spot. This allows for deep penetration into carpeting and full contact coverage of the odor source.
Allow the treated area to sit for a minimum of one hour. The longer it soaks, the more effective it will be. In some cases, soaking overnight might produce optimal results.
Cover the area with a towel and place a weighted object on top. This will help extract any leftover liquid from the affected area.
After the area has dried, vacuum it and check to see if the stain and odor are gone. If they're still present, do the procedure again. Note that older stains or tough odors may need more than one treatment.

Dissolves Stain & Odor Particles Rather Than Covering Them Up
Amaziing Solutions Pet Stain & Odor Remover Enzyme Cleaner
What We Like About It
Introducing the Amaziing Solutions Pet Stain and Odor Remover! This cleaner is specially formulated with powerful cleaning enzymes that quickly remove stains and neutralize odors. Simply spray on the affected area and let the cleaner do its work. You’ll see the stain fade away in no time.
This enzyme cleaner is great for preventing future stains and odors. It’s ideal for use on furniture, clothing, litter boxes, pet carriers, kennels, and more. Use it regularly to help maintain a fresh and clean home for your beloved pet.
This enzyme-activated cleaning formula is the key to an effective clean. The bio-enzyme formula works by dissolving stain and odor particles, rather than just masking them. This ensures long-lasting freshness in your home.
One Minute Pet Tips
To use, remove any solid waste with a hand towel. Then rinse the area affected with warm water and blot dry. Apply this product generously to the area, letting it soak for 10-15 minutes.
This is perfect for use on carpets, upholstery, clothing, flooring, litter boxes, and more. It's safe for use around children and pets, and is backed by a 100% satisfaction guarantee.

Light & Pleasant Citrus Scent
Nature's Miracle Stain & Odor Remover Trigger Spray
What We Like About It
This powerful spot cleaner is perfect for removing stains and odors immediately on contact. The light citrus scent will leave your home smelling clean and fresh, while the enzymatic formula continues working as long as any bio-based mess is still present. Trust the Nature's Miracle brand to get the job done right because it works. Safe for pets and homes, this fast-acting formula clings to surfaces to break down and remove embedded stains and odors.
One Minute Pet Tips
This high-strength formula is ideal for removing those tough stains and odors, quickly and easily. Simply wipe up as much of the mess as possible, then soak the area thoroughly. It is essential for this product to have high saturation so that it can reach the odor source quickly.
Allow the product to work for 10 minutes, then wipe it away. If you want complete odor elimination, leave the treated area wet and let it air dry. The odor will fade as the product dries.
If the stain is old, severe, or difficult to remove, you may need to scrub it lightly with a soft brush for one minute. Then, let it dry for 24 hours.
What We Like About It
This bio-enzymatic odor eliminator is specially formulated to tackle stains and odors caused by pets, and it's tough on everything from urine and feces to vomit and other organic matter. It's safe to use on a wide variety of surfaces, so you can trust it in any room of your house.
It's also safe to use around pets and kids, and is certified by the Carpet and Rug Institute. They offer a 100% satisfaction guarantee if you're not happy with the results, we'll give you a full refund.
One Minute Pet Tips
The powerful formula is specifically designed to remove dog and cat urine odors from carpets, mattresses, upholstery, and more. It's also great for use on hardwood floors, tile, and other surfaces. We feel it belongs on our list of the best five enzyme cleaners for dog urine stains and odors.

Can Be Used For Laundry Pre-Treat
Simple Green Bio Dog Active Stain & Odor Remover
What We Like About It
Simple Green's Bio Enzyme Cleaner & carpet stain remover is a powerful, natural cleaner that uses the power of enzymes to break down stains and odors quickly and easily. It's perfect for use on all kinds of surfaces, including carpets, rugs, pet bedding, upholstery, clothing, and more. And it's safe to use around pets, so you can keep your home clean and fresh without worrying about harming your furry friends.
One Minute Pet Tips
Saturate the soiled area and allow the formula to remain on the surface until dry. For tougher stains and odors, cover the area with a clean white cloth and allow it to sit for 4-5 hours. You'll see the stain absorb into the towel. Repeat as necessary until the stain is gone. Once dry, simply damp-rinse with ambient water to restore shine. For large carpet areas, mix 3 ounces of formula per gallon of water and use it in an extraction machine.
Keep this product away from children. Always wash your hands after using, and avoid contact with eyes or skin surfaces where you have applied the product as it may cause irritation.
Enzyme Cleaner For Dog Urine FAQs
It can be challenging to decide which of the many enzyme cleaners advertised for removing dog urine stains and odors is best. Many of them, including the five we review here, will do a good job. The reality is, most of these cleaners are similar to each other. Some have a better scent, and some are more economical, but you can hardly go wrong with any of them.
Below, we have answered some of the most frequently asked questions about enzyme cleaners for dog urine, to help you make a more informed decision.
Is vinegar an enzymatic cleaner for dog urine?
Yes, vinegar is an enzymatic cleaner for dog urine. It helps to break down the uric acid crystals that are formed when dog urine dries. Uric acid crystals are what cause the bad odor associated with dog urine.
Vinegar also helps to neutralize the ammonia smell that is often associated with dog urine. For best results, mix a 50/50 solution of vinegar and water and pour it over the area where the dog has urinated. Let the solution soak in for a few minutes before blotting it up with a cloth or paper towel. Repeat as necessary until the smell is gone.
Do enzyme cleaners really work on dog urine?
Yes, enzyme cleaners can be effective at removing dog urine. Urine contains enzymes that break down proteins, so the enzymatic cleaner will work to break down the proteins in the urine and neutralize the scent.
It's important to note that not all enzyme cleaners are created equal - some work better than others. So it might take a little trial and error to find the right product for your needs. But with a little patience, you should be able to find an enzymatic cleaner that successfully removes dog urine smells from your home.
How do you get rid of dog urine smell permanently?
There are a few things you can do to get rid of dog urine smell permanently. One is to use an enzymatic cleaner specifically designed to break down pet urine and remove the odor. Another is to make sure you clean the area thoroughly and dry it completely before applying any type of odor eliminator. And you can try keeping your dog on a regular potty schedule so that he eliminates outdoors as often as possible, which will help reduce the amount of urine that accumulates in any one spot.
Is vinegar better than enzyme cleaner?
Vinegar has been used as a cleaning agent for centuries. It's a natural disinfectant and degreaser, and it can be used to clean surfaces, windows, and appliances. Vinegar is also effective at removing water deposits (hard water stains) from glass and porcelain surfaces.
Enzyme cleaner is made from plant-based enzymes that break down organic matter. It's effective at removing protein, starch, grease, and blood stains from surfaces. An enzyme cleaner is also biodegradable and non-toxic, making it a safe choice for cleaning upholstery, carpets, pet accidents, and baby messes.
It depends on what you're trying to clean. If you're dealing with a protein-based stain, like blood or vomit, an enzyme cleaner is your best bet. If you're trying to remove a water deposit or hard water stain, vinegar will probably work better. And if you're just looking for a general-purpose cleaner, either one will work fine.
Can I make my own enzymatic cleaner?
Yes, you can make your own enzymatic cleaner at home using natural ingredients. To make an enzymatic cleaner, you will need white vinegar, baking soda, water, and lemon juice. Mix together 1 cup of white vinegar with 1/2 cup of baking soda in a bowl or container. Next, add 1/2 cup of warm water and stir until the baking soda has dissolved. Add in 2 tablespoons of lemon juice and stir again. Now you are good to go.
How long does it take urine enzyme cleaner to work?
It can take anywhere from a few hours to a day or two for the enzyme cleaner to work. The amount of time it takes for the cleaner to work will depend on how severe the stain is, as well as how long it's been sitting there.
If you're dealing with a fresh stain, then the enzyme cleaner should work within a few hours. If you're dealing with an old, set-in stain, then it may take up to two days for the cleaner to break down the enzymes in the urine and remove the stain completely.
What is considered enzyme cleaners?
Enzyme cleaners are considered a green alternative to traditional harsh chemical-based cleaners. They are made with natural enzymes that break down the bonds of dirt, grease, and stains. This allows the particles to be easily rinsed away with water.
Enzyme cleaners can be used on all types of surfaces, including floors, tiles, countertops, appliances, and carpets. They are safe for people and pets and are biodegradable. Enzyme cleaners can also be used to remove pet urine and feces odors.
Do enzyme cleaners make urine smell worse?
It's no secret that urine can have a strong, unpleasant smell. When left untreated, it can permeate carpeting, furniture, and clothing, making your home or office feel unclean. Enzyme cleaners are often touted as the best way to remove urine stains and odors, but some people believe that they actually make the smell worse.
The truth is that enzyme cleaners can be very effective at removing urine stains and odors, but they need to be used correctly. If you use too much cleaner or don't rinse the area thoroughly afterward, the enzymes can continue to work on the urine, breaking it down into even more smelly compounds. Enzyme cleaners are not always compatible with other cleaning products.
If you use an enzyme cleaner on a stain that has already been treated with another cleaner, it can actually make the smell worse. For best results, always test a small hidden area before using an enzyme cleaner on a large stain.
Are enzyme cleaners worth it?
When it comes to cleaning up tough messes, enzyme cleaners are often touted as being the most effective option. But are they really worth the extra cost? Enzyme cleaners work by breaking down complex molecules into simpler ones, which makes them very effective at removing things like food stains and grease.
However, enzymatic cleaners can also be dangerous if not used properly. Inhaling or coming into contact with concentrated enzyme cleaner can cause irritation to the lungs and skin. As a result, enzyme cleaners should be used with caution, and only on tough messes that cannot be cleaned with simpler methods.
What can I use instead of enzymes?
You can use a variety of natural ingredients to remove stains. For example, club soda can be used to remove red wine stains, white vinegar can be used to remove coffee and tea stains, and baking soda can be used to remove grease or oil stains.
If you don't have any of these ingredients on hand, you can also try using an enzyme cleaner. Enzyme cleaners are designed to break down organic material, so they can be a great option for removing stubborn stains. Just make sure to test the enzyme cleaner on an inconspicuous area of the fabric first to make sure it doesn't cause any damage.
Is bleach or vinegar better for dog urine?
When it comes to cleaning up dog pee, there are a number of different options to choose from. Bleach and vinegar are two of the most popular choices, but it can be difficult to know which one is right for your needs. Vinegar is a natural disinfectant and can be used safely on a variety of surfaces. However, it is important to diluted vinegar before using it, as undiluted vinegar can damage some surfaces.
Bleach is also an effective disinfectant, but it should be used with caution as it can be toxic if inhaled or ingested. When cleaning up dog urine, it is important to identify the type of surface you are working with and select the cleaner that is best suited for that surface. With a little bit of care, you can safely and effectively remove dog urine using either bleach or vinegar.
Is enzyme cleaner toxic to dogs?
The toxicity of an enzyme cleaner will depend on the specific ingredients in the cleaner. However, some enzyme cleaners may contain enzymes that are derived from bacteria or fungi, so they could potentially be toxic to dogs if ingested. It's always best to err on the side of caution and avoid using any type of cleaner if there is any doubt about its toxicity. Instead, consult with your veterinarian about safe and effective ways to clean up after your dog.
What is a heavy-duty enzyme cleaner?
A heavy-duty enzyme cleaner is a cleaner that contains enzymes that break down organic matter. Enzymes are proteins that catalyze (or increase the rate of) chemical reactions in the body. They are found in all living things and can be used to break down waste, grease, and other organic materials.
Heavy-duty enzyme cleaners are used to clean tough stains and messes. They are often used in industrial settings to clean grease and oil spills, but they can also be used in the home to clean carpets, floors, and appliances. Enzyme cleaners are safe to use on most surfaces and are biodegradable.
What do professional carpet cleaners use for pet urine?
There are a few different things that professional carpet cleaners might use to remove pet messes. One option is an enzyme cleaner, which breaks down the proteins in the urine and helps to eliminate the odor.
Another option is a detergent or ammonia-based cleaner, which will help to lift the stain and neutralize the odor. In some cases, a carpet cleaner may also use a steam cleaner to help rinse out the area and remove any traces of urine.
Which cleaners are enzyme cleaners?
Enzyme cleaners are those that contain an enzyme that breaks down the dirt and grime on a surface. The most common type of enzyme cleaner is laundry detergent, which contains the enzyme protease that breaks down proteins in stains.
There are also a number of commercial enzymatic cleaners available that target specific types of dirt or grime. For example, enzymatic pet-urine cleaners break down the proteins in urine that cause the smell, while oven cleaners contain enzymes that break down the burnt-on grease and food residue.
One Minute Recommendation
We hope you find this information helpful and that it makes cleaning up after your pup a little bit easier. We feel that any of the five enzyme cleaners we reviewed will do a decent job for you.
We appreciate you taking the time to stop by, and hope to see you again soon!